Human IFNα Analyte Overview

Interferon alpha (IFN⍺) is a member of the interferon type I family of immune system proteins that bind to the INF⍺/β receptor complex. IFN⍺ plays a role in innate viral immunity and inflammatory responses. It is produced primarily by dendritic cells and leukocytes, however its production is inhibited by interleukin 10. There are multiple subtypes of IFN⍺ that each play unique roles in the immune system, including activating natural killer (NK) cells, stimulating B cell proliferation, promoting the production of human leukocyte antigen (HLA) types I and II, activating dendritic cells to promote an immune response. The interleukin has been found to limit the proliferation of cancer cells by halting the cell cycle and inducing apoptosis. IFN⍺ is commonly produced as a therapeutic under the name interferon alfa to treat a number of cancers and viral diseases, including leukemia, melanoma, and hepatitis B and C infections.

Sample	Concentration (pg/ml)	%CV
Sample 1	169	6%
Sample 2	123	4%
Sample 3	125	5%

Intra-assay (n=20)
Average Intra-assay CV: 5% 

Dilution Factor	% Recovery
	Serum 1	Serum 2	EDTA Plasma 1	EDTA Plasma 2
2	108%	94%	94%	101%
4	108%	104%	97%	95%
8	104%	98%	98%	96%

Average percent linearity: 100%
Percent linearity range: 94 – 108%