NHP IL-2Rα Analyte Overview
NHP IL-2Rα
Also known as CD25, IL-2 receptor alpha chain
Type I transmembrane protein primarily expressed by activated T cells, activated B cells, and regulatory T cells. It can also be detected on myeloid precursors, oligodendrocytes, and resting memory T cells (in humans). IL-2Rα, IL-2Rβ, and the common gamma chain make up the high-affinity IL-2 receptor while a homodimer of the IL-2Rα chain makes up the low-affinity IL-2 receptor. It is important for detecting signal from IL-2 for growth signals, generating immunity, and maintaining immune tolerance.
IL-2Rα can also be found in a soluble form, resulting from extracellular proteolysis. Soluble IL-2Rα may play a role as a decoy receptor that reduces bioavailability of IL-2 or it may promote in trans activation by presenting IL-2 to dimeric IL-2 receptor expressing cells.
Typical Assay Characteristics
| Precision | Linearity | ||
|---|---|---|---|
| Inter-assay | Intra-assay | Range | Average |
| 13% | 12% | 85%-136% | 111% |
Q-Plex™ Details
Species: NHP
Assay Type: Sandwich
Sample Type: Serum/Plasma, Cell Supernatant
Assay Range: 16,000 – 21.95 pg/mL
Lower Limit of Detection (LLD): 10.97 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Features & Requirements
- Data available as fast as 4 hours
- Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
- Requires the Q-View™ Imaging System for analysis of multiplexed chemiluminescent planar-based arrays
Featured Q-Plex Products
Q-Plex™ NHP Anti-Inflammatory Panel 1 (7-Plex)
IL-1RA, IL-2Rα, IL-4, sIL-6R, IL-10, IL-13, TNFRII
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