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Human VEGF Analyte Overview

Human VEGF

Also known as Vascular Endothelial Growth Factor, Vascular Permeability Factor, VPF

Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF), is a family of growth factors that stimulate the formation of new blood vessels. VEGF is critical for vascular development during embryonic growth and supports angiogenesis for wound repair, supporting exercise, and bypassing blocked vessels in adults. The production of VEGF is stimulated by lack of oxygen in a cell, encouraging the production of blood vessels to supply tissues with oxygen and nutrients. VEGF concentrations raise rapidly following injury to support angiogenesis to repair the wound. Abnormal expression of VEGF is implicated in multiple diseases, including rheumatoid arthritis, diabetic retinopathy, age-related macular degeneration, pulmonary emphysema, protein accumulation in the kidneys, and early-onset pre-eclampsia. Tumors that express VEGF are able to vascularize and grow much larger than those that do not and the factor appears to support metastasis, though the exact mechanism is unknown. High levels of VEGF are associated with poor prognosis in cancer patients.
Human VEGF Standard Curve
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Q-Plex™ Details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 2,000 – 2.70 pg/mL
Lower Limit of Detection (LLD):2.70 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 460149HU

Features & Requirements

  • Measure VEGF in 2.25 hours
  • Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
  • Requires the Q-View™ Imaging System for analysis

Assay Performance Characteristics

Precision

Sample Concentration (pg/ml) %CV
Sample 1 483.99 5%
Sample 2 29.46 10%
Sample 3 10.31 4%

Intra-assay (n=20)
Average Intra-assay CV: 6% 

Sample Concentration (pg/ml) %CV
Sample 1 79.61 10%
Sample 2 14.52 7%
Sample 3 32.43 7%

Inter-assay (n=15)
Average Inter-assay CV: 8% 

Dilutional Linearity

Dilution Factor % Recovery
Serum HB Pooled Plasma Serum HG Stripped Serum
2 94% 92% 100% 97%
4 98% 109% 94% 98%
8 95% 73% 103% 95%

Average percent linearity: 98%
Percent linearity range: 91 – 104%

Human VEGF Linearity

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