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Human TNFβ Analyte Overview

Human TNFβ

Also known as TNF beta, Tumor Necrosis Factor Beta, Lymphotoxin-alpha, LT-α

Tumor necrosis factor-beta (TNF beta), also known as lymphotoxin-alpha (LT-α), is a cytotoxic protein of the TNF superfamily, primarily expressed by T and B lymphocytes. As a multifunctional protein, TNFβ mediates a wide range of activities in the innate immune system. TNFβ displays strong anti-prolific abilities and destroys tumor cell lines, making it an important regulator of cancers. It is also involved in regulating cell survival, proliferation, differentiation, and apoptosis. Dysregulation of TNFβ can also lead to tumor promotion when its overproduction leads to a prolonged inflammatory response. TNFβ is critical for normal growth of the gastrointestinal system during embryonic development and maintains an important role in the gut in adults by regulating the immune activity in the intestines. During early development, TNFβ also mediates the formation of the lymph nodes. TNFβ is implicated in the progression of many autoimmune diseases.
human tnfb standard curve
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Q-Plex™ Details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 4,000 – 5.49 pg/mL
Lower Limit of Detection (LLD): 5.29 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 469849HU

Features & Requirements

  • Measure TNFβ in 2.25 hours
  • Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
  • Requires the Q-View™ Imaging System for analysis

Assay Performance Characteristics

Precision

Sample Concentration (pg/ml) %CV
Sample 1 782.91 5%
Sample 2 68.66 6%
Sample 3 21.47 9%

Intra-assay (n=20)
Average Intra-assay CV: 7% 

Sample Concentration (pg/ml) %CV
Sample 1 596.30 8%
Sample 2 33.15 10%
Sample 3 871.31 6%

Inter-assay (n=15)
Average Inter-assay CV: 8% 

Dilutional Linearity

Dilution Factor % Recovery
Cell Culture Supernatant
2 87%
4 96%
8 104%

Average percent linearity: 96%
Percent linearity range: 87 – 104%

human tnfb linearity

Q-Plex Products featuring TNFβ

Human Multiplex Kits

Human Cytokine – Screen (16-plex)

Human Cytokine – Stripwells (16-plex)

Human Cytokine – High Sensitivity (15-plex)

Featured Q-Plex Products

Human Multiplex Kits

Q-Plex™ Human Cytokine HS (15-Plex) 

Q-Plex™ Human Cytokine Inflammation Panel 2 (10-Plex)

Q-Plex™ Human Cytokine Screen (16-Plex)

Q-Plex™ Human Cytokine Stripwells (16-Plex)

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