Human TIMP-2 Analyte Overview

Tissue inhibitor of metalloproteinases 2 (TIMP2) is a glycoprotein in the TIMP family of proteins, which regulate the activity of matrix metalloproteinases. Metalloproteinases degrade the extracellular matrix. As an inhibitor of metalloproteinases, TIMP2 minimizes matrix digestion by these enzymes and is most effective at inhibiting MMP2. TIMP2 also has the unique ability to repress the proliferation of endothelial cells. It may be critical to tissue homeostasis, preventing the overgrowth of certain cell types during blood vessel formation and limiting MMP2 activity in the extracellular matrix of tissues during remodeling. TIMP2 is critical to normal hippocampal behavior and supports cognitive function. It may suppress certain cancers by preventing cell proliferation during angiogenesis.

Sample	Concentration (pg/ml)	%CV
Sample 1	1571.82	5%
Sample 2	84.33	10%
Sample 3	–	–

Intra-assay (n=20)
Average Intra-assay CV: 8%

Dilution Factor	% Recovery
	Stripped Serum	Cell Culture Supernatant	Sample 3	Sample 4
2	82%	106%	Not Detectable	Not Detectable
4	86%	104%
8	88%	109%

Average percent linearity: 98%
Percent linearity range: 91 – 104%