Human TIMP-1 Analyte Overview
Human TIMP-1
Also known as Tissue Inhibitors of Metalloproteinases 1, Erythroid Potentiating Activity, EPA, Human Collagenase Inhibitor, HCI
Tissue inhibitor of metalloproteinases 1 (TIMP1) is a glycoprotein in the TIMP family of proteins, which regulate the activity of matrix metalloproteinases. Metalloproteinases degrade the extracellular matrix. As an inhibitor of metalloproteinases, TIMP-1 minimizes matrix digestion by these enzymes and is most effective at inhibiting MMP1, MMP7, and MMP9. It is produced by a wide range of cell types and likely helps to maintain tissue homeostasis by preventing tissue degradation. It is important for maintaining the extracellular matrix and supporting wound healing. TIMP1 also plays a critical role in pregnancy, facilitating implantation of the egg onto the uterine wall and regulating early transcription in the developing embryo. High levels of TIMP1 are associated with poor prognosis in cancer patients.
Q-Plex™ Details
Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 12,697 – 17.39 pg/mL
Lower Limit of Detection (LLD): 12.95 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 469549HU
Features & Requirements
- Measure TIMP-1 in 2.25 hours
- Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
- Requires the Q-View™ Imaging System for analysis
Assay Performance Characteristics
Precision
| Sample | Concentration (pg/ml) | %CV |
| Sample 1 | 1571.82 | 5% |
| Sample 2 | 84.33 | 10% |
| Sample 3 | – | – |
Intra-assay (n=20)
Average Intra-assay CV: 8%
| Sample | Concentration (pg/ml) | %CV |
| Sample 1 | 8533.89 | 9% |
| Sample 2 | 99.77 | 14% |
| Sample 3 | 34.23 | 12 |
Inter-assay (n=15)
Average Inter-assay CV: 12%
Dilutional Linearity
| Dilution Factor | % Recovery | |||
| Stripped Serum | Cell Culture Supernatant | Sample 3 | Sample 4 | |
| 2 | 82% | 106% | Not Detectable | Not Detectable |
| 4 | 86% | 104% | ||
| 8 | 88% | 109% | ||
Average percent linearity: 98%
Percent linearity range: 91 – 104%
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