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Human RANTES Analyte Overview

Human RANTES

Also known as regulated on activation normal T cell expressed and secreted cysteine-rich protein, CCL5, XCP1

Regulated on activation, normal T cell expressed and secreted (RANTES), also known as Chemokine (C-C motif) ligand 5 (CCL5) is a cytokine that mediates inflammatory response of the immune system. It is chemotactic to T lymphocytes, basophils, and eosinophils. RANTES activates and increases the cytotoxicity and proliferation of natural killer cells. It is secreted by fibroblasts, platelets, and T cells at the site of inflammation. RANTES is also involved in the progression and metastasis of some malignant tumors where it may promote migration of cancer cells. Its expression is upregulated in the white adipose tissue of obese individuals and may be involved in obesity related inflammation. RANTES is strongly HIV suppressive, preventing replication of certain HIV strains and is currently under investigation as a therapeutic for the virus.
Human RANTES Standard Curve
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Q-Plex™ Details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 13,071 – 17.93 pg/mL
Lower Limit of Detection (LLD): 6.59 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 468949HU

Features & Requirements

  • Measure RANTES in 2.25 hours
  • Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
  • Requires the Q-View™ Imaging System for analysis

Assay Performance Characteristics

Precision

Sample Concentration (pg/ml) %CV
Sample 1 524.33 10%
Sample 2 31.80 6%
Sample 3 12.26 5%

Intra-assay (n=20)
Average Intra-assay CV: 7%

Sample Concentration (pg/ml) %CV
Sample 1 835.34 4%
Sample 2 12.81 11%
Sample 3 499.43 6%

Inter-assay (n=15)
Average Inter-assay CV: 7% 

Dilutional Linearity

Dilution Factor % Recovery
Serum HA Stripped Serum Cell Culture Supernatant Serum HF
2 110% 103% 110% 89%
4 111% 95% 99% 107
8 98% 102% 103% 99%

Average percent linearity: 102%
Percent linearity range: 89 – 111%

Human il-10 linearity

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