Human MCP-2 Analyte Overview

Monocyte chemoattractant protein 2 (MCP-2), also known as Chemokine (C-C motif) ligand 8 (CCL8), is a chemokine involved in early stages of an immune responses. It interacts with cellular receptors CCR1, CCR2B, and CCR5 to attract and activate many types of immune cells, including monocytes, T-cells, and natural killer cells during and immune response and mast cells, eosinophils, and basophils in the case of an allergic response. MCP-2 is often expressed by stromal tissues that have been exposed to pathogens. It recruits immature monocytes to sites of inflammation to mature. Since MCP-2 demonstrates high affinity binding to receptor CCR5, a common HIV co-receptor, it has the potential to be HIV protective. It is often found in high levels in patients experiencing metastasis of a tumor and may play a role in promoting metastasis.

Intra-assay (n=20)
Average Intra-assay CV: 6%

Sample	Concentration (pg/ml)	%CV
Sample 1	140.42	7%
Sample 2	14.23	6%
Sample 3	5.19	5%

 

Average percent linearity: 103%
Percent linearity range: 79 – 122%

Dilution Factor	% Recovery
	Cell Culture Supernatant	Activated Plasma	Pooled Serum	Serum HF
2	98%	111%	110%	101%
4	105%	89%	111%	107%
8	101%	79%	98%	122%