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Human IL-5 Analyte Overview

Human IL-5

Also known as Interleukin 5, Eosinophil differentiation factor, TRF, BCDF, B-cell differentiation factor I, Eo-CSF

Interleukin 5 (IL-5) is a cytokine that modules immune system activities. It is secreted by activated TH2 cells, mast cells, eosinophils, EBV-transformed B cells, Reed-Sternberg cells (found in Hodgkin’s Disease) and iNKT cells. It increases immunoglobulin secretion, stimulates B-cell growth, and promotes eosinophilic and basophilic white blood cell differentiation and activation, as well as increasing mobilization of eosinophils from bone marrow. In a healthy person, IL-5 supports immune responses to multicellular parasites through eosinophil proliferation. IL-5 is implicated in diseases that are characterized by high levels of eosinophils, particularly severe allergic diseases, such as hay fever and asthma.
Human IL-5 Standard Curve
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Q-Plex™ Details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 3,000 – 4.12 pg/mL
Lower Limit of Detection (LLD): 2.9 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 466349HU

Features & Requirements

  • Measures IL-5 in 2.25 hours
  • Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
  • Requires the Q-View™ Imaging System for analysis

Assay Performance Characteristics

Precision

Intra-assay (n=20)
Average Intra-assay CV: 5%

Sample Concentration (pg/ml) %CV
Sample 1

337.69

 4%
Sample 2 23.07 4%
Sample 3 9.05 8%

 

Inter-assay (n=15)
Average Inter-assay CV: 10%

Sample Concentration (pg/ml) %CV
Sample 1 399.09 7%
Sample 2 129.41 12%
Sample 3 11.29 10%

 

Dilutional Linearity

Average percent linearity: 95%
Percent linearity range: 87 – 102%

Dilution Factor % Recovery
Serum HA Serum HB Serum HC Pooled Plasma
2 99% 87% 91% 99%
4 95% 95% 95% 102%
8 94% 93% 98% 91%
human il-4 linearity

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