Human IL-1β Analyte Overview
Human IL-1β
Also known as Interleukin 1 beta, IL1β, leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor, pre-interleukin 1 beta, catabolin
Interleukin 1 beta (IL-1β) is a cytokine involved in pro-inflammatory responses. It is produced by activated macrophages, natural killer cells, monocytes, and neutrophils in response to wounds, infections, or microbial endotoxins and is processed to its active state by caspase 1. It mediates inflammatory responses and plays a role in cell proliferation, apoptosis, and differentiation. IL-1β inflammation within the central nervous system is known to result in pain and hypersensitivity. High levels of IL-1β are associated with autoinflammatory diseases, particularly the group of rare heterozygous dominant diseases known as cryopyrin-associated periodic syndromes, that often affect the skin, joints, eyes, and nervous system. Inhibition of IL-1β treats these diseases and eases symptoms in common conditions such as type 2 diabetes, cardiac failure, gout, and rheumatoid arthritis. Dysregulated IL-1β is associated with tumor formation in a wide variety of cancers.
Q-Plex™ Details
Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma, Herparin Plasma
Assay Range: 12,226 – 21.80 pg/mL
Lower Limit of Detection (LLD): 10.90 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 465549HU
Features & Requirements
- Measure IL-1β in 2.25 hours
- Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
- Requires the Q-View™ Imaging System for analysis
Assay Performance Characteristics
Precision
Intra-assay (n=20)
Average Intra-assay CV: 6%
| Sample | Concentration (pg/ml) | %CV |
| Sample 1 | 1333.12 | 6% |
| Sample 2 | 103.03 | 7% |
| Sample 3 | 35.49 | 6% |
Inter-assay (n=15)
Average Inter-assay CV: 8%
| Sample | Concentration (pg/ml) | %CV |
| Sample 1 | 2821.92 | 5% |
| Sample 2 | 1091.31 | 8% |
| Sample 3 | 175.1 | 10% |
Dilutional Linearity
Average percent linearity: 94%
Percent linearity range: 86 – 103%
| Dilution Factor | % Recovery | |||
| Serum HA | Serum HB | Serum HC | Pooled Plasma | |
| 2 | 92% | 95% | 86% | 91% |
| 4 | 100% | 95% | 103% | 98% |
| 8 | 94% | 98% | 91% | 87% |
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