Human IL-12p70 Analyte Overview
Human IL-12p70
Also known as IL-12, Interleukin 12, Active Heterodimer of Interleukin-12
Q-Plex™ Details
Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 3,000 – 5.3 pg/mL
Lower Limit of Detection (LLD): 2.65 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 460449HU
Features & Requirements
- Measure IL-12p70 in 2.25 hours
- Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
- Requires the Q-View™ Imaging System for analysis
Assay Performance Characteristics
Precision
Intra-assay (n=20)
Average Intra-assay CV: 7%
| Sample | Concentration (pg/ml) | %CV |
| Sample 1 |
570.17 |
9% |
| Sample 2 | 50.53 | 5% |
| Sample 3 | 15.66 | 8% |
Inter-assay (n=15)
Average Inter-assay CV: 7%
| Sample | Concentration (pg/ml) | %CV |
| Sample 1 | 55.64 | 9% |
| Sample 2 | 410.85 | 7% |
| Sample 3 | 704.21 | 6% |
Dilutional Linearity
Average percent linearity: 103%
Percent linearity range: 98 – 110%
| Dilution Factor | % Recovery | |||
| Serum HH | Pooled Plasma | Pooled Serum | Serum HF | |
| 2 | 105% | 107% | 98% | 108% |
| 4 | 100% | 102% | 100% | 103% |
| 8 | 108% | 100% | 99% | 110% |
Featured Q-Plex Products
Human Multiplex Kits
Not Finding What You Need?
Customize a Kit
Not seeing the biomarker or analyte you need? Customize your kit! The Q-Plex™ Custom Assays are fully quantitative ELISA-based Chemiluminescent assay kits allowing the concurrent measurement of up to 18 biomarkers or analytes.
Send in Your Samples
Choose up to 18 different biomarkers that will work best for your research. Send in your samples and let us run them for you.
Contact a Representative
Have questions about the kit or interested in more information? Contact one of our representative to get all your questions answered.
As researchers and problem solvers ourselves, we understand the value of sound data. We are proud to be a part of research that can better the world. We value the relationships, partnerships, and friendships that we have built with the people who trust and use our technology. We are committed to building these relationships. You can count on us to answer the phone and take time to thoroughly address questions or concerns about any of our products. In an industry that is reputed for grandiose claims, we trust that our quality standards and our customer service set us apart from the competition. If you’re happy with our products or if you think we can do something better, we hope you will let us know.