Human CXCL-1 Analyte Overview
Human CXCL-1
Also known as Chemokine Ligand 1, KC, CINC-1, Neutrophil-activating protein 3 (NAP-3), GRO1, Melanoma Growth Stimulating Activity alpha (MGSA-α)
CXCL-1 or GROα, is a proinflammatory chemokine that plays a role in immune responses and many cancers. In the healthy immune system, CXCL-1/GROα is produced by monocytes, epithelial cells, and fibroblasts in response to inflammatory activity. It induces neutrophil migration thought chemotaxis and has antimicrobial effects. It is also capable of promoting lymphatic tube and blood vessel formation within the circulatory system. In individuals with chronic inflammatory diseases, it is produced in high levels by many cell types. Abnormally high levels of CXCL-1 stimulates the growth of tumors and is particularly associated with melanomas due to its normal expression in epithelial cells. As a frequent oncogenic culprit, it is often found up-regulated when tumor cells are present throughout the body.
Q-Plex™ Details
Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 2,000 – 2.74 pg/mL
Lower Limit of Detection (LLD): 1.20 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 464249HU
Features & Requirements
- Measure CXCL-1 in 2.25 hours
- Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
- Requires the Q-View™ Imaging System for analysis
Assay Performance Characteristics
Precision
| Sample | Concentration (pg/ml) | %CV |
| Sample 1 | 368.24 | 10% |
| Sample 2 | 25.47 | 9% |
| Sample 3 | 8.60 | 7% |
Intra-assay (n=20)
Average Intra-assay CV: 9%
| Sample | Concentration (pg/ml) | %CV |
| Sample 1 | 719.47 | 6% |
| Sample 2 | 51.27 | 8% |
| Sample 3 | 9.97 | 10% |
Inter-assay (n=15)
Average Inter-assay CV: 8%
Dilutional Linearity
| Dilution Factor | % Recovery | |||
| Stripped Serum | Cell Culture Supernatant | Pooled Serum | Serum HA | |
| 2 | 100% | 106% | 100% | 107% |
| 4 | 99% | 102% | 108% | 102% |
| 8 | 105% | 105% | 89% | 94% |
Average percent linearity: 101%
Percent linearity range: 89 – 108%
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