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Human FGF Analyte Overview

Human FGF

Also known as Human Basic Fibroblast Growth Factor, bFGF, FGF2, FGF-β

Basic Fibroblast Growth Factor (FGF-β, FGF-2) is a growth factor involved in the early development and maintenance of populations of embryonic and adult stem cells. During embryonic development it is critical for the pluripotency and self-renewal of embryonic stem cells, functioning to promote stem cell division and limit cellular differentiation. In adults is found in the basement membranes and extracellular matrix of blood vessels in many tissues, including neural, pituitary, adrenal cortex, corpus luteum, and placental tissues where it modulates wound healing, tissue repair, and neuronal function or degeneration. It has been demonstrated to be protective to heart tissue during a heart attack. Low levels of FGF-β are associated with anxiety disorders. FGF-β is upregulated in response to tumors and inflammation stimuli and is implicated in tumor growth.
Human FGF Standard Curve
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Q-Plex™ Details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 5,000 – 6.90 pg/mL
Lower Limit of Detection (LLD): 1.27 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 463549HU

Features & Requirements

  • Maesure FGF in 2.25 hours
  • Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
  • Requires the Q-View™ Imaging System for analysis

Assay Performance Characteristics

Precision

Sample Concentration (pg/ml) %CV
Sample 1 734.33 4%
Sample 2 56.47 9%
Sample 3 75.33 13%

Intra-assay (n=20)
Average Intra-assay CV: 9%

Sample Concentration (pg/ml) %CV
Sample 1 60.66 10%
Sample 2 693.19 9%
Sample 3 143.70 15%

Inter-assay (n=15)
Average Inter-assay CV: 11%

Dilutional Linearity

Dilution Factor % Recovery
Pooled Plasma Pooled Serum Serum HE
2 108% 113% 102%
4 118% 95% 85%
8 96%

Average percent linearity: 103%
Percent linearity range: 85 – 118%

Human FGF Linearity

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