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Human Eotaxin Analyte Overview

Human Eotaxin-1

Also known as Eosinophil Chemotactic Protein, Chemokine ligand 11 (CCL11)

Eotaxins are a group of three cytokines, eotaxin-1, eotaxin-2, and eotaxin-3, that share similar structure and function. Eotaxins chemoattract eosinophils, which they are capable of activating and regulating during an immune response. Eotaxins have the unique ability to selectively prime eosinophils for chemotaxis, direct their migration/chemotaxis, and activate inflammatory activity in the cells attracted. Due to their role in attracting and activating eosinophils, eotaxins are implicated in allergies and eczema. High levels of eotaxin-1 are associated with aging and schizophrenia. Eotaxin-1 is also often found upregulated in cannabis users. Eotaxin-2 is also a chemoattractant for T lymphocytes and neutrophils. Its concentration is associated with risk and severity of asthma symptoms.
human eotaxin standard curve
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Q-Plex™ Details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 1,745 – 2.40 pg/mL
Lower Limit of Detection (LLD): 0.73 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 463449HU

Features & Requirements

  • Measure Eotaxin in 2.25 hours
  • Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
  • Requires the Q-View™ Imaging System for analysis

Assay Performance Characteristics

Precision

Sample Concentration (pg/ml) %CV
Sample 1 462.94 7%
Sample 2 29.68 7%
Sample 3 11.79 7%

Intra-assay (n=20)
Average Intra-assay CV: 7%

Sample Concentration (pg/ml) %CV
Sample 1 104.45 7%
Sample 2 896.61 5%
Sample 3 35.92 11%

Inter-assay (n=15)
Average Inter-assay CV: 8% 

Dilutional Linearity

Dilution Factor % Recovery
Serum HD Pooled Plasma Pooled Serum Stripped Serum
2 116%
4 109% 116% 115% 120%
8 89% 101% 100% 101%

Average percent linearity: 107%
Percent linearity range: 89 – 120%

Human eotaxin linearity

Featured Q-Plex Products 

Q-Plex™ Human Chemokine (9-Plex)

The Q-Plex Human Chemokine (9-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of Eotaxin, GROa, I-309, IL-8, IP-10, MCP-1, MCP-2, RANTES, and TARC.

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