Human Ang-2 Analyte Overview
Human Ang-2
Also known as Angiopoietin-2, ANGPT2
Angiopoietin2 (Ang-2) is a glycoprotein that is involved in the growth of new blood vessels and inflammation. Ang-2 is expressed heavily during early development, but in healthy adults is typically only expressed in the uterus and ovaries of females and during wound healing and inflammatory responses. During pregnancy Irregular levels of Ang-2 in the mother are associated with cases of pre-eclampsia, ectopic implantation, and Down Syndrome. For this reason, Ang-2 is often measured to predict at-risk-pregnancy. Ang-2 is implicated in cancer, during which the protein restricts blood vessels in expanding tumors, causing the regression and loss of vessels at the center of a tumor. Patients with a fever that are also expressing high levels of Ang-2 are at greater risk for the development of septic shock.
Ang-2 Q-Plex™ Details
Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma, Heparin
Assay Range: 10,000 – 13.70 pg/mL
Lower Limit of Detection (LLD): 3.9 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 462349HU
Features & Requirements
Measure Ang-2 in 2.25 hours
Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
Requires the Q-View™ Imaging System for analysis
Assay Performance Characteristics
Precision
| Sample | Concentration (pg/ml) | %CV |
| Sample 1 | 1976.76 | 5% |
| Sample 2 | 13% | |
| Sample 3 | 57.25 | 4% |
Intra-assay (n=20)
Average Intra-assay CV: 7%
| Sample | Concentration (pg/ml) | %CV |
| Sample 1 | 781.95 | 9% |
| Sample 2 | 931.09 | 8% |
| Sample 3 | 1834.96 | 9% |
Inter-assay (n=15)
Average Inter-assay CV: 9%
Dilutional Linearity
| Dilution Factor | % Recovery | |||
| Serum HA | Serum HH | Serum HD | Pooled Plasma | |
| 2 | 100% | 93% | 92% | 80% |
| 4 | 103% | 103% | 90% | 90% |
| 8 | 108% | 103% | 95% | 88% |
Average percent linearity: 97%
Percent linearity range: 88 – 111%
Q-Plex Products Featuring Ang-2
Q-Plex™ Human Angiogenesis (9-Plex)
The Q-Plex Human Angiogenesis (9-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of Ang-2, FGF, HGF, IL-8, PDGF-BB, TIMP-1, TIMP-2, TNFα, and VEGF.
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Not seeing the biomarker or analyte you need? Customize your kit! The Q-Plex™ Custom Assays are fully quantitative ELISA-based Chemiluminescent assay kits allowing the concurrent measurement of up to 18 biomarkers or analytes.
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