Q-Plex™ Rat Cytokine Inflammation (9-Plex)
Q-Plex Rat Cytokine Inflammation (9-Plex)
For measurement of IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12p70, TNFα
- Species Specificity: Rat
- Validated Sample Type: Cell Culture Supernatant, Serum, Plasma
- Sample Volume Required: Min. 25 µL
- Estimated Assay Length: 3 hours (180 minutes)
- Intra-Assay Cross-Reactivity: All analytes <1% intra-panel cross-reactivity
- Assay Type: Planar based multiplexed ELISA
- Detection Method: Chemiluminescent
- Multiplex Format: 96-well solid plate
- Catalog #: 111649RT
| Analyte | Assay Type | Calibrator Range | Upper Limit of Quantification (ULOQ) | Lower Limit of Quantification (LLOQ) | Limit of Detection | Precision (Inter-assay) | Precision (Intra-assay) | Average Linearity |
|---|---|---|---|---|---|---|---|---|
| IFNγ | Sandwich | 6,000 - 8.23 (pg/mL) | 4,200 (pg/mL) | 10.7 (pg/mL) | 4.12 (pg/mL) | 9.8% | 9.7% | 97% |
| IL-1α | Sandwich | 5,400 - 7.41 (pg/mL) | 3,780 (pg/mL) | 9.6 (pg/mL) | 3.7 (pg/mL) | 8.5% | 8.8% | 101% |
| IL-1β | Sandwich | 3,200 - 4.39 (pg/mL) | 2,240 (pg/mL) | 5.7 (pg/mL) | 2.19 (pg/mL) | 7.7% | 7.9% | 102% |
| IL-2 | Sandwich | 9,800 - 13.44 (pg/mL) | 6,860 (pg/mL) | 17.5 (pg/mL) | 6.72 (pg/mL) | 9.9% | 10.1% | 99% |
| IL-4 | Sandwich | 4,000 - 5.49 (pg/mL) | 2,800 (pg/mL) | 7.1 (pg/mL) | 2.74 (pg/mL) | 9.4% | 4.3% | 97% |
| IL-6 | Sandwich | 83,000 - 114 (pg/mL) | 58,100 (pg/mL) | 148 (pg/mL) | 56.93 (pg/mL) | 8.0% | 9.1% | 104% |
| IL-10 | Sandwich | 14,000 - 19.20 (pg/mL) | 9,800 (pg/mL) | 25 (pg/mL) | 9.6 (pg/mL) | 10.8% | 8.4% | 101% |
| IL-12p70 | Sandwich | 92,000 - 126 (pg/mL) | 64,400 (pg/mL) | 164.1 (pg/mL) | 63.1 (pg/mL) | 10.5% | 7.1% | 101% |
| TNFα | Sandwich | 37,000 - 50.75 (pg/mL) | 25,900 (pg/mL) | 66 (pg/mL) | 25.38 (pg/mL) | 9.4% | 8.4% | 116% |
Product Overview
The Q-Plex™ Rat Cytokine Inflammation (9-Plex) is a fully quantitative ELISA-based Chemiluminescent assay allowing the concurrent measurement of nine biomarkers or analytes. Using just 25 µL of sample per well, up to 80 samples can be assayed for all nine markers in the panel within 3 hours.
The Q-Plex Rat Cytokine – Inflammation (9-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Q-Plex™ plates are built by adsorbing nine distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.
Product Documentation
Kit Content
Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.
Reagents Include:
- Calibrator
- Detection Mix
- Substrate
- Sample Diluents
- Streptavidin HRP
- Wash Buffer
Processing
All Quansys Q-Plex™ Multiplex and Singleplex assays require the use of the Q-View™ software to read and interpret the test results. A fully-functional, free trial version of the software is available to download, install, and use to analyze your first kit(s). At the end of the trial period, a purchased license is required to continue the use of the Q-View software.
Q-Plex arrays are developed and optimized to work with Q-View imagers. Quansys does not guarantee the results obtained from other imagers and not all imagers are compatible with Quansys Q-Plex arrays.
Other Equipment Recommendations
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