Q-Plex™ Human Type 2 Immunity (5-Plex)
Q-Plex™ Human Type 2 Immunity (5-Plex)
For measurement of IL-4, IL-5, IL-10, IL-13, IL-33
- Species Specificity: Human
- Validated Sample Type: Human Serum, EDTA/Heparin Plasma
- Sample Volume Required: Min. 25 µL
- Estimated Assay Length: 2.25 hours
- Intra-Assay Cross-Reactivity: No significant interference observed. <1% cross-reactivity observed.
- Assay Type: Sandwich
- Detection Method: Chemiluminescent
- Multiplex Format: 96-well solid plate
- Catalog #: 114233HU
| Analyte | Assay Type | Calibrator Range | Upper Limit of Quantification (ULOQ) | Lower Limit of Quantification (LLOQ) | Limit of Detection | Precision (Inter-assay) | Percision (Intra-assay) | Average Linearity |
|---|---|---|---|---|---|---|---|---|
| IL-4 | Sandwich | 1,676 – 2.30 (pg/mL) | 1,173 (pg/mL) | 3.0 (pg/mL) | 1.15 (pg/mL) | 6% | 6% | 94% |
| IL-5 | Sandwich | 3,000 – 4.12 (pg/mL) | 2,100 (pg/mL) | 5.3 (pg/mL) | 2.06 (pg/mL) | 10% | 7% | 95% |
| IL-10 | Sandwich | 8,039 – 11.03 (pg/mL) | 5,627 (pg/mL) | 14.3 (pg/mL) | 5.51 (pg/mL) | 7% | 5% | 103% |
| IL-13 | Sandwich | 3,000 – 4.12 (pg/mL) | 2,100 (pg/mL) | 5.3 (pg/mL) | 2.06 (pg/mL) | 6% | 7% | 98% |
| IL-33 | Sandwich | 350 - 0.48 (pg/mL) | 245 (pg/mL) | 0.62 (pg/mL) | 0.24 (pg/mL) | 15% | 6% | 92% |
Product Overview
The Q-Plex™ Human Type 2 Immunity (5-Plex) is a fully quantitative plate-based multiplex ELISA for the detection of cytokines associated with type 2 immunity.
Functions of type 2 immunity include protecting the host, maintaining metabolic homeostasis, suppressing type 1 inflammation, barrier defense, and tissue regeneration. Like other immune responses, excessive or chronic activation of type 2 immunity can be harmful to the host. Allergic disease, inability to defend against pathogens, and fibrosis have been described as harmful actions of type 2 immunity.
Producers of type 2 cytokines include macrophages, mast cells, T cells, macrophages, monocytes, basophils, eosinophils, and natural killer cells. A variety of immune and non-immune cells have been noted to produce type 2 cytokine responses in response epithelial damage, release of alarmins, and antigen presentation/recognition.
Product Documentation
Kit Content
Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.
Reagents Include:
- Detection Mix
- Substrates
- Sample Diluents
- Streptavidin HRP
- Wash Buffer
Processing
All Quansys Q-Plex™ Multiplex and Singleplex assays require the use of the Q-View™ software to read and interpret the test results. A fully-functional, free trial version of the software is available to download, install, and use to analyze your first kit(s). At the end of the trial period, a purchased license is required to continue the use of the Q-View software.
Q-Plex™ arrays are developed and optimized to work with Q-View™ imagers. Quansys does not guarantee the results obtained from other imagers and not all imagers are compatible with Quansys Q-Plex arrays.
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