Q-Plex™ Bovine Cytokine Panel 1 (5-Plex)
Q-Plex Bovine Cytokine Panel 1 (5-Plex)
For measurement of IFNγ, IL-4, IL-10, IL-12p40, TNFα
- Species Specificity: Bovine, African Buffalo Syncerus caffer
- Validated Sample Type: Serum, plasma, and cell culture supernatant
- Sample Volume Required: Min 25µL (varies)
- Estimated Assay Length: 3.5 hours
- Assay Type: Sandwich
- Detection Method: Chemiluminescent
- Multiplex Format: 96-Well Solid Plate
- Catalog #: 112349BV
| Analyte | Assay Type | Calibrator Range | Upper Limit of Quantification (ULOQ) | Lower Limit of Quantification (LLOQ) | Limit of Detection | Precision (Inter-assay) | Precision (Intra-assay) | Average Linearity |
|---|---|---|---|---|---|---|---|---|
| IFNγ | Sandwich | 1,650 - 2.26 (pg/mL) | 1,155 (pg/mL) | 2.94 (pg/mL) | 1.13 (pg/mL) | 14.1% | 4.9% | 110% |
| IL-4 | Sandwich | 500 - 0.69 (pg/mL) | 350 (pg/mL) | 0.89 (pg/mL) | 0.34 (pg/mL) | 11.7% | 3.0% | 107% |
| IL-10 | Sandwich | 5,000 - 6.86 (pg/mL) | 1,166 (pg/mL) | 8.92 (pg/mL) | 3.43 (pg/mL) | 9.8% | 3.0% | 108% |
| IL-12p40 | Sandwich | 200,000 - 274 (pg/mL) | 140,000 (pg/mL) | 356 (pg/mL) | 137 (pg/mL) | 12.1% | 6.8% | 100% |
| TNFα | Sandwich | 3,000 - 4.12 (pg/mL) | 2,100 (pg/mL) | 16.05 (pg/mL) | 2.06 (pg/mL) | 12.9% | 4.5% | 115% |
Product Overview
The Q-Plex™ Bovine Cytokine Panel 1 (5-Plex) is a fully quantitative plate-based multiplex ELISA for the detection of cytokines associated with inflammation.
Production of inflammatory cytokines occurs normally to protect the host from pathogens, pain and wound healing, and growth signaling. However, excessive, or chronic production of inflammatory cytokines can damage host tissues and is associated with certain autoimmune diseases and cancers.
Product Documentation
Kit Content
Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.
Reagents Include:
- Sample Diluent
- Calibrator
- Detection Mix
- Streptavidin HRP
- Wash Buffer
- Substrates
Processing
All Quansys Q-Plex™ Multiplex and Singleplex assays require the use of the Q-View™ software to read and interpret the test results. A fully-functional, free trial version of the software is available to download, install, and use to analyze your first kit(s). At the end of the trial period, a purchased license is required to continue the use of the Q-View software.
Q-Plex arrays are developed and optimized to work with Q-View imagers. Quansys does not guarantee the results obtained from other imagers and not all imagers are compatible with Quansys Q-Plex arrays.
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Not seeing the biomarker or analyte you need? Customize your kit! The Q-Plex™ Custom Assays are fully quantitative ELISA-based Chemiluminescent assay kits allowing the concurrent measurement of up to 18 biomarkers or analytes.
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