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Human MCP-1 Analyte Overview

Human MCP-1

Also known as Monocyte Chemotactic Protein 1, Chemokine Ligand 2, CCL2, Small Inducible Cytokine A2, SCYA2

Monocyte chemoattractant protein 1 (MCP1), also known as chemokine (C-C motif) ligand 2 (CCL2) and small inducible cytokine (A2MCP-1), is a chemokine that plays a role in the body’s response to tissue injury and infection. It is produced by many cell types in response to tissue damage, and is a chemoattractant to monocytes, basophils, memory T cells, and dendritic cells. MCP-1 is commonly found at the site of tooth and bone injuries or infections, where it is expressed by osteoclasts and osteoblasts. It is also produced by cells of the nervous system and is implicated in many inflammatory neurological disorders. Elevated MCP-1 levels are often associated with sepsis, Crohn’s disease, lupus nephritis, amyotrophic lateral sclerosis, multiple sclerosis, rheumatoid arthritis, acute pancreatitis, and atherosclerosis. MCP-1 is also upregulated in several cancers including gastric carcinoma, esophageal squamous cell carcinoma, malignant glioma, and ovarian, pancreatic, bladder, and breast cancers.
human MCP-1 standard curve
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Q-Plex™ Details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 2,000 – 2.74 pg/mL
Lower Limit of Detection (LLD): 2.60 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 467049HU

Features & Requirements

  • Measure MCP-1 in 2.25 hours
  • Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
  • Requires the Q-View™ Imaging System for analysis

Assay Performance Characteristics

Precision

Intra-assay (n=20)
Average Intra-assay CV: 7%

Sample Concentration (pg/ml) %CV
Sample 1

325.97

 7%
Sample 2 19.72 5%
Sample 3 8.37 9%

 

Inter-assay (n=15)
Average Inter-assay CV: 7%

Sample Concentration (pg/ml) %CV
Sample 1 345.54 7%
Sample 2 23.93 6%
Sample 3 8.99 7%

 

Dilutional Linearity

Average percent linearity: 108%
Percent linearity range: 96 – 118%

Dilution Factor % Recovery
Cell Culture Supernatant Activated Plasma Pooled Serum Serum HF
2 96% 111% 118% 111%
4 106% 109% 115% 107%
8 102% 112% 100% 112%
human MCP-1 linearity

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