Human IFNγ Analyte Overview
Human IFNγ
Also known as IFN gamma interferon gamma, type II interferon
Interferon gamma (IFNγ), also known as Type II interferon, is a cytokine that plays a critical role in the innate and adaptive immune systems. It serves as protection against viral infections, as well as some bacterial and protozoal infections, and plays a role in tumor control. It is capable of inhibiting viral replication and works to modulate immunological responses to multiple kinds of pathogens, with effects including promoting natural killer cell activity, activating macrophages to destroy foreign cells, increasing antigen presentation, and inducing the expression of numerous immune defense factors. IFNγ is primarily produced by natural killer and natural killer T cells during the innate immune response and by CD4 and CD8 cytotoxic T lymphocyte effector T cells as part of an adaptive response. It has been shown to halt growth and induce apoptosis in some types of tumors. During pregnancy, IFNγ aids in the remodeling of the uterine wall and placenta to accommodate a zygote.
Q-Plex™ Details
Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 8,000 – 10.97 pg/mL
Lower Limit of Detection (LLD): 4.95 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 464649HU
Features & Requirements
- Measures IFNγ in 2.25 hours
- Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
- Requires the Q-View™ Imaging System for analysis
Assay Performance Characteristics
Precision
| Sample | Concentration (pg/ml) | %CV |
| Sample 1 | 53.60 | 13% |
| Sample 2 | 66.58 | 16% |
| Sample 3 | 57.16 | 15% |
Intra-assay (n=20)
Average Intra-assay CV: 6%
| Sample | Concentration (pg/ml) | %CV |
| Sample 1 | 181.74 | 8% |
| Sample 2 | 150.08 | 9% |
| Sample 3 | 226.86 | 9% |
Inter-assay (n=15)
Average Inter-assay CV: 8%
Dilutional Linearity
| Dilution Factor | % Recovery | |||
| Cell Culture Supernatant | Pooled Plasma | Pooled Serum | Stripped Serum | |
| 2 | 99% | 94% | 95% | 99% |
| 4 | 101% | 104% | 95% | 98% |
| 8 | 102% | 90% | 94% | 92% |
Average percent linearity: 97%
Percent linearity range: 90 – 104%
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