Human IL-2 Analyte Overview
Human IL-2
Also known as Interleukin 2
Q-Plex™ Details
Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 5,213 – 7.15 pg/mL
Lower Limit of Detection (LLD): 4.12 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 465749HU
Features & Requirements
- Measure IL-2 in 2.25 hours
- Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
- Requires the Q-View™ Imaging System for analysis
Assay Performance Characteristics
Precision
Intra-assay (n=20)
Average Intra-assay CV: 5%
| Sample | Concentration (pg/ml) | %CV |
| Sample 1 | 510.82 | 4% |
| Sample 2 | 35.56 | 4% |
| Sample 3 | 12.15 | 7% |
Inter-assay (n=15)
Average Inter-assay CV: 6%
| Sample | Concentration (pg/ml) | %CV |
| Sample 1 | 1916.94 | 6% |
| Sample 2 | 139.65 | 10% |
| Sample 3 | 488.43 | 3% |
Dilutional Linearity
Average percent linearity: 99%
Percent linearity range: 82 – 110%
| Dilution Factor | % Recovery | |||
| Serum HA | Serum HB | Serum HC | Pooled Plasma | |
| 2 | 87% | 93% | 82% | 93% |
| 4 | 108% | 101% | 103% | 107% |
| 8 | 107% | 110% | 98% | 97% |
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