Human Adiponectin Analyte Overview
Human Adiponectin
Also known as GBP-28, apM1, AdipoQ, Acrp30
Adiponectin is a protein hormone generated by adipose tissue that is involved in glucose regulation and fatty acid breakdown. Though the hormone is produced by fat cells, its levels are reduced in obese individuals and increase with caloric restriction and weight reduction. In lean individuals, the adiponectin is produced by adipose tissue in bone marrow. Decreased adiponectin levels are associated with metabolic disorders, including non-alcoholic fatty liver disease, obesity, type 2 diabetes, insulin resistance, and atherosclerosis, as well as some cancers. Studies suggest that the hormone promotes the synthesis of HDL cholesterol and low levels of adiponectin are associated with high triglyceride counts. Treatment with adiponectin has been demonstrated to reverse insulin resistance in mice and administration of a combination of leptin and adiponectin has been shown to trigger weight loss.
Adiponectin Q-Plex™ Details
Species: Human
Assay Type: Competitive
Sample Type: Urine
Assay Range: 28,000 – 38 ng/mL
Lower Limit of Detection (LLD): 31.5 ng/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 462149HU
Features & Requirements
- Measure Adiponectin in 2.25 hours
- Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
- Requires the Q-View™ Imaging System for analysis
Assay Performance Characteristics
Precision
| Sample | Concentration (pg/ml) | %CV |
| Sample 1 | 38212.00 | 5% |
| Sample 2 | 1964.95 | 7% |
| Sample 3 | 564.35 | 7% |
Intra-assay (n=20)
Average Intra-assay CV: 6%
| Sample | Concentration (pg/ml) | %CV |
| Sample 1 | 496.6 | 15% |
| Sample 2 | 11750.5 | 6% |
| Sample 3 | 18620.2 | 7% |
Inter-assay (n=15)
Average Inter-assay CV: 10%
Dilutional Linearity
| Dilution Factor | % Recovery | |||
| Serum 1 | Serum 2 | EDTA Plasma 1 | EDTA Plasma 2 | |
| 2 | 105% | 109% | 100% | 90% |
| 4 | 105% | 111% | 92% | 89% |
| 8 | 97% | 93% | 91% | 88% |
Average percent linearity: 97%
Percent linearity range: 88 – 111%
Not Finding What You Need?
Customize a Kit
Not seeing the biomarker or analyte you need? Customize your kit! The Q-Plex™ Custom Assays are fully quantitative ELISA-based Chemiluminescent assay kits allowing the concurrent measurement of up to 18 biomarkers or analytes.
Send in Your Samples
Choose up to 18 different biomarkers that will work best for your research. Send in your samples and let us run them for you.
Contact a Representative
Have questions about the kit or interested in more information? Contact one of our representative to get all your questions answered.
As researchers and problem solvers ourselves, we understand the value of sound data. We are proud to be a part of research that can better the world. We value the relationships, partnerships, and friendships that we have built with the people who trust and use our technology. We are committed to building these relationships. You can count on us to answer the phone and take time to thoroughly address questions or concerns about any of our products. In an industry that is reputed for grandiose claims, we trust that our quality standards and our customer service set us apart from the competition. If you’re happy with our products or if you think we can do something better, we hope you will let us know.