NHP MIF Analyte Overview
NHP MIF
Also known as macrophage migration inhibitory factor
Macrophage migration inhibitory factor (MIF) is a cytokine whose function was initially described to inhibit macrophage migration. However, it has since been described to promote migration and recruitment of leukocytes to sites of inflammation. Produced mainly by macrophages and B- and T-cells but can also be produced by numerous other cell types. It signals through CD74 and can promote cell survival by inhibiting activation induced apoptosis as well as counter-regulate glucocorticoid suppression of immune responses.
Typical Assay Characteristics
| Precision | Linearity | ||
|---|---|---|---|
| Inter-assay | Intra-assay | Range | Average |
| 20% | 4% | 67%-161% | 113% |
Q-Plex™ Details
Species: NHP
Assay Type: Sandwich
Sample Type: Serum/Plasma, Cell Supernatant
Assay Range: 230,000 – 315.5 pg/mL
Lower Limit of Detection (LLD): 158 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Features & Requirements
- Data available as fast as 4 hours
- Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
- Requires the Q-View™ Imaging System for analysis of multiplexed chemiluminescent planar-based arrays
Featured Q-Plex Products
Q-Plex™ NHP Chemokine Screen (9-Plex)
CXCL-1, IL-8, IP-10, MCP-1, MIF, MIG, MIP-1α, MIP-1ß, RANTES
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