Q-Plex™ Human Cytokine HS (15-Plex)
Q-Plex Human Cytokine HS (15-Plex)
For measurement of IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-15, IL-17A, IL-23, TNFα, TNFβ
- Species Specificity: Human
- Validated Sample Type: Serum, EDTA/Heparin Plasma
- Sample Volume Required: Min. 25 µl
- Estimated Assay Length: 4.83 hours
- Intra-Assay Cross-Reactivity: All analytes < 1% intra-panel cross-reactivity
- Assay Type: Planar Based Multiplexed ELISA
- Detection Method: Chemiluminescent
- Multiplex Format: 96-well solid plate
- Catalog #: 112433HU
| Analyte | Assay Type | Calibrator Range | Upper Limit of Quantification (ULOQ) | Lower Limit of Quantification (LLOQ) | Limit of Detection | Precision (Inter-assay) | Precision (Intra-assay) | Average Linearity |
|---|---|---|---|---|---|---|---|---|
| IFNγ | Sandwich | 99 – 0.14 (pg/mL) | 69.3 (pg/mL) | 0.18 (pg/mL) | 0.068 (pg/mL) | 10% | 5% | 99% |
| IL-1α | Sandwich | 1,333 – 1.83 (pg/mL) | 933 (pg/mL) | 2.38 (pg/mL) | 0.91 (pg/mL) | 9% | 6% | 102% |
| IL-1β | Sandwich | 4,075 – 5.59 (pg/mL) | 2852 (pg/mL) | 7.27 (pg/mL) | 2.79 (pg/mL) | 11% | 4% | 103% |
| IL-2 | Sandwich | 64 – 0.088 (pg/mL) | 44.8 (pg/mL) | 0.11 (pg/mL) | 0.044 (pg/mL) | 8% | 6% | 101% |
| IL-4 | Sandwich | 21 – 0.029 (pg/mL) | 14.7 (pg/mL) | 0.04 (pg/mL) | 0.014 (pg/mL) | 11% | 8% | 97% |
| IL-5 | Sandwich | 333 – 0.46 (pg/mL) | 233 (pg/mL) | 0.59 (pg/mL) | 0.23 (pg/mL) | 10% | 8% | 102% |
| IL-6 | Sandwich | 333 – 0.46 (pg/mL) | 233 (pg/mL) | 0.59 (pg/mL) | 0.23 (pg/mL) | 8% | 6% | 91% |
| IL-10 | Sandwich | 2,680 – 3.68 (pg/mL) | 1,876 (pg/mL) | 4.78 (pg/mL) | 1.84 (pg/mL) | 11% | 3% | 99% |
| IL-12p70 | Sandwich | 3,000 – 4.12 (pg/mL) | 2,100 (pg/mL) | 5.35 (pg/mL) | 2.06 (pg/mL) | 12% | 10% | 99% |
| IL-13 | Sandwich | 333 – 0.46 (pg/mL) | 233 (pg/mL) | 0.59 (pg/mL) | 0.23 (pg/mL) | 11% | 5% | 105% |
| IL-15 | Sandwich | 3,578 – 4.91 (pg/mL) | 2,504 (pg/mL) | 6.38 (pg/mL) | 2.45 (pg/mL) | 8% | 11% | 102% |
| IL-17A | Sandwich | 5,589 – 7.67 (pg/mL) | 3912 (pg/mL) | 9.97 (pg/mL) | 3.83 (pg/mL) | 9% | 5% | 104% |
| IL-23 | Sandwich | 10,000 – 13.72 (pg/mL) | 6230 (pg/mL) | 57.3 (pg/mL) | 6.86 (pg/mL) | 8% | 10% | 100% |
| TNFα | Sandwich | 661 – 0.91 (pg/mL) | 462 (pg/mL) | 1.18 (pg/mL) | 0.45 (pg/mL) | 12% | 8% | 101% |
| TNFβ | Sandwich | 444 – 0.61 (pg/mL) | 310 (pg/mL) | 0.79 (pg/mL) | 0.30 (pg/mL) | 9% | 7% | 94% |
Product Overview
The Q-Plex Human Cytokine HS (15-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 15 biomarkers or analytes. Using only 25 µL of sample per well, up to 80 samples can be assayed for all 15 markers in the panel within 4.83 hours.
The Q-Plex Human Cytokine HS (15-Plex) provides researchers with an easy-to-use and cost-effective means of generating a cytokine profile for each sample. Q-Plex plates are built by adsorbing 15 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.
Product Documentation
Kit Content
Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.
Reagents Include:
- Calibrator
- Detection Mix
- Substrates
- Sample Diluents
- Streptavidin HRP
- Wash Buffer
Processing
All Quansys Q-Plex™ Multiplex and Singleplex assays require the use of the Q-View™ software to read and interpret the test results. A fully-functional, free trial version of the software is available to download, install, and use to analyze your first kit(s). At the end of the trial period, a purchased license is required to continue the use of the Q-View software.
Q-Plex arrays are developed and optimized to work with Q-View imagers. Quansys does not guarantee the results obtained from other imagers and not all imagers are compatible with Quansys Q-Plex arrays.
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