Q-Plex™ Human Micronutrient v2 (7-Plex)
Q-Plex Human Micronutrient v2 (7-Plex)
For measurement of AGP, CRP, Ferritin, HRP2, RBP4, sTfR, Thyroglobulin
- Species Specificity: Human
- Validated Sample Type: Serum and Heparin Plasma
- Sample Volume Required: 5 µL
- Estimated Assay Length: 3.5 hours
- Intra-Assay Cross-Reactivity: No significant interference observed. <1% cross-reactivity observed.
- Assay Type: Varies (See Performance and Validation Data table)
- Detection Method: Chemiluminescent
- Multiplex Format: 96-well solid plate
- Catalog: 355149HU
| Analytes | Assay Types | Calibrator Range | Upper Limit of Quantification (ULOQ) | Lower Limit of Quantification (LLOQ) | Limit of Detection | Precision (Inter-assay) | Precision (Intra-assay | Average Linearity |
|---|---|---|---|---|---|---|---|---|
| AGP | Competitive | 0.36 - 0.00049 (g/L) | 0.25 (g/L) | 0.0019 (g/L) | 0.00025 (g/L) | 5% | 5% | 102% |
| CRP | Sandwich | 1 - 0.0014 (mg/L) | 0.7 (mg/L) | 0.0018 (mg/L) | 0.00069 (mg/L) | 5% | 7% | 103% |
| Ferritin | Sandwich | 23.1 - 0.032 (µg/L) | 16.2 (µg/L) | 0.041 (µg/L) | 0.016 (µg/L) | 7% | 8% | 87% |
| HRP2 | Sandwich | 1.9 - 0.0026 (µg/L) | 1.33 (µg/L) | 0.010 (µg/L) | 0.0013 (µg/L) | 7% | 5% | 101% |
| RBP4 | Competitive | 1.01 - 0.0014 (µmol/L) | 0.71 (µmol/L) | 0.0054 (µmol/L) | 0.00069 (µmol/L) | 6% | 8% | 95% |
| sTfR | Sandwich | 4 - 0.0055 (mg/L) | 2.8 (mg/L) | 0.0071 (mg/L) | 0.0027 (mg/L) | 10% | 11% | 101% |
| Thyroglobulin | Sandwich | 15.1 - 0.021 (µg/L) | 10.57 (µg/L) | 0.027 (µg/L) | 0.01 (µg/L) | 6% | 11% | 100% |
Principle of the Assay
Micronutrient deficiencies can have systemic and enduring effects on health and wellbeing. If left untreated, they can significantly reduce quality of life and may even have deadly consequences. While micronutrient malnutrition can be found in populations across the globe, it disproportionately affects children and women of reproductive age in low- and middle-income countries. Public health surveillance programs are needed to identify populations at risk, determine the appropriate interventions to apply, and monitor program impacts. The Q-Plex Human Micronutrient v2 (7-plex), developed in partnership with PATH, facilitates micronutrient status surveillance by simplifying laboratory testing and reducing cost.
Performance of the new Q-Plex Human Micronutrient v2 (7-Plex) was optimized to improve precision and recalibrated using samples provided by the Centers for Disease Control Performance Verification Program for Serum Micronutrients as well as the Vital-EQA program. Continued participation in the Vital-EQA program increases confidence in the assay through the elimination of bias.
The Q-Plex Human Micronutrient v2 (7-Plex) is an accurate and affordable tool for measuring micronutrient biomarkers in serum and plasma samples collected as part of related public health surveillance programs. With the ease of use of a traditional ELISA protocol, the platform will help facilitate testing within the survey country.
Product Documentation
Kit Content
Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.
Reagents Include:
- Calibrator
- Competitor
- Detection Mix
- Substrates
- Sample Diluents
- Streptavidin HRP
- Wash Buffer
Processing
Q-Plex arrays are developed and optimized to work with Q-View imagers. Quansys does not guarantee the results obtained from other imagers and not all imagers are compatible with Quansys Q-Plex arrays.
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